大鼠肝过氧化物酶体中脂肪酸的分析

Analysis of Fatty Acids in Rat Liver Peroxisomes

用双 (2 乙基己基 )酚酞酸酯 (DEHP)诱导大鼠肝过氧化物酶体增殖 ,再用蔗糖密度梯度离心法分离大鼠肝细胞过氧化物酶体 ,并用十七烷酸作内标 ,以毛细管气相色谱法在非极性SPB 1石英毛细管柱上对其中的 11种脂肪酸进行分离测定。正常组和诱导组的大鼠肝过氧化物酶体中的不饱和脂肪酸和长链脂肪酸所占总脂肪酸的比例及总脂肪酸的统计结果是 :诱导组的不饱和脂肪酸的含量高于正常组的 (P <0 0 5 ) ,而两个组的脂肪酸总量及长链脂肪酸的含量无明显差别。结果提示 :诱导组的大鼠肝过氧化物酶体的脂肪酸成分发生了变化 ,其膜结构与正常组的不相同。

Peroxisomes of rat liver from normal and di (2 ethylhexyl) phthalate (DEHP) treated animals were isolated by density gradient centrifuge method The fatty acids in the liver peroxisomes were extracted with chloroform and methanol, and were esterified with boron trifluoride and methanol The fatty acids methyl esters were separated on SPB 1(similar to SE 30, OV 101 ) capillary column, and were determined using heptadecanoic acid (C 17∶0 )as an internal standard for the eleven fatty acids (C 10∶0 C 22∶6 )in these peroxisomes by gas chromatography The ratio of unsaturated fatty acids to total fatty acids in the control was significantly less than that in DEHP treated animals ( P <0 05), but there was no difference between the control and DEHP treated animals for the content of total fatty acids and the ratio of long chain fatty acids to total fatty acids The results show that DEHP treatment can alter the fatty acid composition of peroxisome. The membrane structure of the treated peroxisome was different to that of the control