毛细管气相色谱法同时测定鸡蛋中的胆固醇和α-维生素E

Determination of Cholesterol and α-Tocopherol in Eggs by Capillary Gas Chromatography

采用 2 0mol/L的KOH乙醇溶液直接皂化 ,石油醚萃取 ,大口径毛细管气相色谱法测定鸡蛋中的胆固醇和α 维生素E(α VE) ,讨论并确定了鸡蛋样品皂化的最佳条件。胆固醇和α VE的回收率分别为 91.0 %～ 93.3%和82 .5 %～ 95 .0 % ,其RSD分别为 2 7%～ 3 1%和 4 9%～ 6 9% (n =5 )。该法具有简便、快速、灵敏、准确等优点。

A simple and rapid method has been developed for simultaneous determination of cholesterol and α tocopherol in eggs. The method involves alcoholic KOH saponification of the samples,extraction with petroleum ether,and determination by wide bore capillary gas chromatography. The recoveries of cholesterol and α VE in eggs were 91.0% 93.3% and 82.5% 95.0%, with RSDs of 2 7% 3.1%and 4.9% 6 9% respectively.