茶多酚诱导癌细胞凋亡结果的几种快速检测方法比较

Comparison Study on Assaying methods About Effectiveness of Cell Apoptosis Induced by Tea Polyphenols

比较几种常规染色方法在茶多酚体外诱导EJ细胞的凋亡检测的效果。以同剂量茶多酚诱导膀胱癌EJ细胞凋亡,用琼脂糖凝胶电泳的方法确认细胞凋亡,比较苏木精、台盼兰、吉姆萨染色方法在光学显微镜下细胞凋亡形态的检测效果。结果表明,经苏木精、台盼兰、吉姆萨染色方法染色的标本均可在显微镜下找到凋亡细胞形态,而以苏木精染色方法处理的标本染色效果最好。结论是用苏木精染色方法加琼脂糖凝胶电泳是检测茶多酚诱导的膀胱癌EJ细胞凋亡的最佳方法。

In this paper, we compared the hematoxylin- eosin staining method, typan blue staining and giemsa staininmsethods to detectin vitro the EJ cell apoptosis induced by tea polyphenols. DNA gel electrophoresis method was used to make sure of apoptosis result induced by the same concentration of tea polyphenols. Typical cell apoptosis structure “ DNA ladder' was found in the EJ cell culture apoptosis induced by the tea polyphenols￡¨ 500|ì g/ml￡(c) . Typical cell apoptosis structure was all found in the three methods apoptosis cell staining . But the hematoxylin- eosin staining of the apoptosis cell showed more clearly than the other two. The conclusion was that DNA gel electrophoresis method with hematoxylin- eosin staining to detect EJ cell apoptosis induced by tea polyphenols was the best method of the three.