膜蛋白(受体)在膜中的运动测定

Measurement of the Mobility of Protein(Receptor) in the Membrane

自 1970年Frye和Edidin首先用细胞融和方法证实膜蛋白在膜中的运动以来 ,有关测定膜蛋白侧向扩散运动和旋转的各种技术已有详细报道[1,2 ] 。影响运动的因素很多 ,如 :温度、pH ;细胞内其它组分与蛋白质的相互作用 ;细胞内的微丝、微管 ;膜粘度、分子的形状大小等。所有因这些影响而出现的运动都是蛋白质在膜中的自由扩散运动 ,另外一些现象则是受新陈代谢影响的复杂过程 (如带帽现象等 )

Membrane proteins in membrane undergo the lateral diffusion and rotational diffusion. A lot of methods and techniques were used in measuring the mobility of membrane proteins in the membrane. In this paper, we report the measurement of rotational diffusion of membrane protein in membrane using polarized flash photolysis and the lateral diffusion measurement of membrane protein in membrane using fluorescence imaging of charge coupled device. We also got some good results using these methods.