摘要
从江苏省自然腹泻病猪粪便中分离获得的四株猪轮状病毒,都能在MA104单层细胞上形成蚀斑,其中三株形成大小不同的蚀斑,另一株仅形成小蚀斑。蚀斑试验培养瓶,在37℃中培养五天,大蚀斑直径达3—6毫米,小蚀斑达1—2毫米。在灯光下,可见蚀斑呈云块状;在低倍显微镜下,蚀斑呈深色的细胞团块;用10％福尔马林结晶紫液固定染色,活细胞层染成深紫色而病毒感染的细胞脱落而成蚀斑。
Four strains of porcine rotavirus isolated from the feces of diarrheal piglets in Jiangsu Province were cultivated successfully and formed distinguishable plaques in the continuous Rhesus monkey cell line(MA104). Three of the four strains(Na86, Ning71, Jiang150) usually yielded both large and small plaques, whereas the other one(Li-99) induced only small plaques. After incubated at 37℃ for 5 days the large plaques measured 3-6mm and small ones 1-2mm in diameter. The plaques look like cloudy clot under light and appear dark cell lumps under low power microscope. After the treatment of formalin and crystal violet, the living cells stained deepviolet and the cells infected with virus detached from the glass wall and the plaques were formed.
基金
科学基金会资助
