乙型肝炎病毒前S蛋白的研究——Ⅵ.前S蛋白与人血清白蛋白的结合

Researches on Pre-S Proteins of Hepatitis B Virus Ⅵ. Binding of Pre-S Proteins with Human Serum Albumin

本文观察了40份HBV感染者血清前S_1、前S_2与PHSAR的关系,结果表明前S_2可能是PHSA的主要结合部位,但前S_1可能也具有结合PHSA的活性。体外条件下PHSA可阻断前S_1、前S_2以及PHSAR活性,这种阻断效应与PHSA稀释度呈反相关系。本文还证实,HBV感染者血(?)内存在HBV外壳蛋白—HSA复合物,推测HSA与HBV外壳蛋白的结合可能是宿主抗HBV感染的免疫防御方式之一。

40 serum samples were used to study the relation of Pre-S_1 and pre-S_2 proteins to polymerized human serum albumin receptor (PHSAR). Our results suggested that pre-S_2 may be the main binding site for PHSA, but pre-S_1 may also possess the activity to bind PHSA. Pre-S_1, pre-S_2 and PHSAR may be blocked in vitro by pretreatment with PHSA and the negative correlation between the blocking rate and the dilution of PHSA was seen. We also showed that the complexes of HBV envelop protein-human serum albumin may occur in the serum with HBV infection and it is proposed that the binding between HSA and HBV envelop proteins may be one of mechinisms for host to defence HBV infection.