小球藻的异养生长及培养条件优化

The hetertrophic culture of Chlorella and optimization of growth condition

对小球藻异养培养中的碳源、氮源、微量元素—镁离子以及其他培养条件的影响进行了探讨 ,并测定了小球藻的生长曲线。优化结果 :C∶ N为 4∶ 1～ 5∶ 1 ,硫酸镁的量为 1 g/L;培养条件为 :p H6～ 7,接种量1 0 % ,温度 3 0°C。在此条件下 ,异养培养小球藻 ,其 OD值可达 1 8,蛋白质为 3 0 % ,叶绿素含量为 1 .2 %。

The effect of carbon sources, nitrogen sources, microelement-Mg 2+ and culture condition for heterotrophic culture of Chlorella was researched. We also get the growth curve. The results of optimization:C/N is 4∶1～5∶1, MgSO 4 is 1g/L; pH is 6～7, inoculation is 10%, temperature is 30 °C. Under this condition, the OD is 18, protein concentration is 30%, chlorophyll concentration is 1.2%.