土壤细菌16SrRNA基因变异型及其与植被的相关研究

Molecular genetic variation of soil bacteria as determined by polymorphism of restrictive fragment length of 16SrRNA genes and its relation w ith vegetation shift

绕过细菌的分离培养 ,直接提取土壤DNA ,扩增、克隆土壤细菌群体的 16S核糖体RNA基因 (16SrD NA) .根据该基因各种变异类型的限制性片段长度多型性 ,分析土壤细菌分子遗传多样性及其与植被的相互关系 .植被的改变影响土壤养分 ,进而改变土壤细菌群落结构 .土壤细菌遗传多样性和分化能反映植被的变化 .

Bacterial 16S ribosomal RNA (hereafter rDNA) genes were PCR amplified from soil DNA,and cloned without artificial cultivation.The molecular genetic diversity a nd its relation with vegetation shift were determined by RFLP of randomly select ed recombinants containing rDNA inserts.Soils under different vegetation rear re lative bacterial community by formulating their physical and chemical components .The molecular genetic diversity and its differentiation could serve as the appr opriate indicators of vegetation shifts.