双阳梅花鹿三个EST序列的发现

Identification of Three ESTs of Shuangyang Sika Deer

采用HSV 1 2TK和人IL 1 β特异性引物分别对鹿肝组织cDNA文库及鹿血cDNA进行PCR扩增 ,得到的特异性cDNA片段分别连入TA克隆载体测序 ,得到三个EST ,输入GeneBank、Swissprot、dbEST等数据库中进行同源比较分析。同源分析表明 :获得双阳梅花鹿三个基因EST。EST 1为梅花鹿α2 抗纤溶酶同源基因EST ,EST 2为梅花鹿水通道蛋白同源基因EST ,EST 3为鹿血未知基因EST ,并含有一个 2 67个碱基编码

We amplified the sika deer hepatic tissue cDNA library through PCR with HSV1-2 TK specific primer and obtained a cDNA fragment. Another two cDNA fragments of 500*!bp and 1*!000*!bp were also isolated from sika deer blood cDNA through RT PCR primed with a human IL 1β specific primer. These cDNA fragments were cloned into pGEM T Easy plasmid. Sequencing and BLAST search revealed that EST 1 is homologous to α 2 antiplasmin, EST 2 is homologous to aquaporin 4 (AQP 4), and EST 3 is a novel EST of sika deer containing a 267*!bp ORF.