中华绒螯蟹组织中一株类志贺邻单胞菌的分离与特性分析

ISOLATION AND CHARACTERISTICS OF PLESIOMONAS SHIGELLOIDES FROM TISSUE OF ERIOCHEIR SINENSIS

从江苏省启东市某水产养殖场患病的中华绒螯蟹组织中 ,分离到一株类志贺邻单胞菌 ,定名为JE- 1株。该菌株为革兰阴性短杆菌 ,具端生单鞭毛 ,主要生化特性为氧化酶阳性 ,发酵葡萄糖产酸不产气 ,发酵麦芽糖、乳糖、肌醇等 ;不发酵甘露醇、鼠李糖、阿拉伯糖等 ;触酶、精氨酸双水解酶、鸟氨酸脱羧酶、赖氨酸脱羧酶阳性。对小鼠、鹌鹑、鲫鱼血细胞无溶血性 ,最适生长条件为温度 2 8℃ ,p H7,含 1% Na Cl。经健康蟹回感 ,未见发病。

A strain was isolated from tissue of crabs in a certain aquacuature farm in Qidong city.The strain showed following characteristics:Gram-negative rod,motile,oxidase positive,glucose,maltose,lactose,inositol fermentative.but not mannitol,rhamnose,arabinos fermentative,H 2O 2,arginine,ornithine,lysine positive,hemolysis negative.These fcatures indicated that isolate belong to Plesiomonasshigelloides, and named JE-1.The best of appropriate environment is 28℃, pH 7 and 1% NaC1.As the result of infection test in crabs,the strainis not pathogenic bacterium.