三种方法检测淋球菌的结果分析

THE RESULT OF THREE MEFHODS DETECTED GONOCOCCDS

用革兰染色、淋球菌培养、PCR检测三种方法同时对 135例泌尿生殖道患者进行淋球菌检测。革兰染色阳性 5 3例 ,检出率 39.2 6 % ,淋球菌培养 6 8例 ,检出率 5 0 .37% ,PCR阳性 78例 ,检出率5 7.78%。上述结果经统计学处理 x2 =9.39,P<0 .0 5。革兰染色与淋球菌培养和 PCR检测有差异 ,淋球菌培养与 PCR检测 P>0 .0 5 ,二种方法差异无显著性 ,其中 ,用 PCR法测淋球菌敏感、特异、快速。提示临床上在革兰染色、淋球菌培养均阴性而症状又符合淋球菌感染者考虑可用 PCR方法检测。这对淋病患者进行准确地早期发现 ,早期诊断和防治及因淋球菌漏检而引起的淋病传播起到重要作用。

Gonococcus was detected in 135 patients of unrinary tract infec tion with Grain's stain,gonococcus culture and PCR,the results have shown th at 53( 39 26%)patients detected wih Gram's stain were positive,68(50 37%)patients pos itive with culture and 78(57 78%)patients positive with PCR.Statistical compari sons were made with x~ 2 test.P<0 05 was accepted as significant be tween Gr am's stain and gonococcus culture,but P>0 05 has shown that gonococcus culture and PCR detection have no significantly differed.However,PCR detection was sens ilve,pecularily and saving time,when a patient was doubted with gonococcus infec tion but was negative with Gram's stain and culture,PCR is considered to be impo rtant effective of early finding,diaganosis,treatment and in timely prevention.