红树DNA导入茄子获得耐盐性后代的研究

Introducing total DNA of Rhizothora apicularta to Solanum melongena to generate Salt-tolerant progenies

将海滩耐盐植物红树DNA经花粉管通道导入茄子 ,其后代在海滩试种 ,用海水直接浇灌 ,筛选出耐盐性转化株 ,约 90 %能开花结果 ,完成生长周期 ,并对其在盐胁迫下的生长情况、蒸腾速率、光合速率、磷酸烯醇式丙酮酸羧化酶酶活以及叶片气孔的电镜观察等进行了研究。实验结果表明 ,通过花粉管通道导入红树DNA培育的茄子 。

Total DNA from Rhizophora apiculata, salt\|resistant, seashore\|grown plant,was introduced into Solanum melongena via pollen tubes.The D 2 progenies were planted on the beach,watered directly by seawater.Salt\|tolerant transformed plant were selected under the pressure of saline.Approximately 90% of the transformed plants were examined,including the growth,rate of transpiration and photosynthesis,the activity of PEPCase and the morphology of the blade stomas.The studies have shown that it is feasible to generate salt\|tolerant transformed plants by introducing the total DNA of Rhizophora apiculata. \;