摘要
用微注射法将鸡的PGCs注入到麻鸭的胚盘下腔中 ,用鸡W染色体DNA探针通过原位杂交对供体细胞在嵌合体胚胎中的发育作了研究。 5 4个胚胎各器官都有不同程度的嵌合 ,其中肝脏的嵌合率最高 ,性腺最低。胚盘细胞移植法可制备鸡 -麻鸭的体细胞和种系嵌合体。
Chicken W chromosome-specific DNA fragments were obta in ed after transformation, extraction, purification and digestion with EcoRⅠ of pUGD1201. The W chromosome-specific DNA probe was a 1.2kb insert, which was labelled by the random-technique using a Digoxigenin Labeling Kit. Chicken PGCs from a line of White Leghorns were microinjected into Maya duck blastoderms at stage Ⅹ. The embryos at 6,8,9,12,16,18 days of incubation were used to study th e development of chicken PGCs in the chimeric embryos. Used chicken W chromosome -specific DNA probes by in situ hybridization, development of donor cells i n chicken-duck chimeric embryos were studied. In 54 chimeric embryos, there wer e different degrees of chimerism in the organs. The chimeric rate of the livers was the highest, which of the gonads was the lowest. It is demonstrated that som atic and germline chimeras can be yielded successfully by transferring stage Ⅹ chicken blastodermal cells.
