马桑内酯致痫大鼠海马神经元丢失及化学性质的研究

STUDY OF NEURONAL LOSS AND CHEMICAL CHARACTER IN HIPPOCAMPUS OF THE CONVULSIVE RATS INDUCED BY CORCIARIA LACTONE

为观察癫痫发作过程海马神经元丢失细胞的主要死亡方式 ,用马桑内酯建立慢性致痫大鼠模型 ,以 Nissl染色、免疫组化、TUNEL法以及后二者相结合的技术 ,对海马神经元及其中的抑制性神经元的丢失进行了分区观察、统计、分析。结果发现 :致痫组海马各区神经元较对照组明显减少 ,不同区域减少程度不同 ,以齿状回减少最为明显 ;免疫组化结果显示 ,致痫组的抑制性神经元 GABA-IR神经元、PV-IR神经元、CB-IR神经元在海马各区有不同程度的减少 ,其中 GABA-IR神经元减少最明显。在海马各区所有丢失的神经元中 ,三种抑制性神经元所占的比例不同 ;并在海马各区均可见到 TUNEL -GABA、TUNEL-PV、TU NEL-CB双重反应阳性神经元。提示 :马桑内酯所致的癫痫发作 ,可引起海马神经元丢失 ,且不同区域丢失的程度不同 ,丢失的神经元可能以凋亡方式为主 ;海马抑制性神经元 GABA-IR、PV-IR、CB-IR神经元在癫痫发作过程中也有不同程度的丢失 。

To explore the pathogenesis of epilepsy, the chronic epileptic rat models induced by Corciaria Lactone (CL) were established to observe the decrease pattern and their chemical characteristics of the hippocampal neurons by means of Nissl staining, immunohistochemistry method, TUNEL technique and TUNEL co immunohistochemistry double labeling technique in this study. All kinds of positive neurons in each region of hippocampus and dentate gyrus were analyzed statistically. The main results were: (1) Compared with the control group, number of cells in hippocampal formation of convulsive group significantly decreased, and the degree of cell loss was different in different regions: the neuronal loss of dentate gyrus was the most obvious. (2) The GABA IR, CB IR, PV IR neurons in different regions of hippocampal formation decreased differently. Loss of GABA IR positive cells was the most obvious among three kinds of immunoreactive cells. (3) TUNEL positive neurons appeared in all regions of hippocampal formation. In dentate gyrus, the amount of TUNEL positive cells was the most obvious. (4) The TUNEL GABA, TUNEL PV, TUNEL CB double labeling cells could be found in all areas of hippocampus. It suggests that the seizure induced by CL causes cell loss in hippocampus, and the degree of cell loss is different in different region. Apoptosis may be the chief pattern of cell loss in hippocampus of the convulsive rats induced by CL. The inhibitory GABA IR, CB IR, PV IR neurons decrease with different degree in different areas of hippocampus during the episode, and some of these inhibitory cells also decrease with apoptosis.