二硫键寡核苷酸Microarray用于丙型肝炎病毒检测及HCV1b分型的研究

Study on the Application of Disulfide Bonds Oligonucleotide Arrays in Detecting Hepatitis C Virus and Genotyping HCV1b

利用二硫键法制作寡核苷酸 ＤＮＡ Ｍｉｃｒｏａｒｒａｙ，并将该技术应用到 ＨＣＶ的检测中，建立了一种快速、灵敏、安全的寡核苷酸 ＤＮＡ Ｍｉｃｒｏａｒｒａｙ丙肝病毒检测体系．该体系还可同时对占中国丙肝病毒 ７５％左右的ＨＣＶ１ｂ亚型进行分型．另外，在丙肝病毒基因组 ＲＮＡ反转录的过程中采用随机引物，从而为进一步的多种肝炎病毒的寡核苷酸 ＤＮＡ Ｍｉｃｒｏａｒｒａｙ混合检测奠定了基础．

A simple, safe and quick method mostly convenient for laboratory and clinical use of HCV detection was de- veloped using oligoneucleotide arrays made by the disulfide bonds method. These arrays can genotype HCV1b, that is dominant in china. Furthermore, the random primers were used in reverstranscribing HCV genome, a foundation for developing multi-virus detection and genotyping method using arrays.