CO_(2)激光治疗尖锐湿疣烟雾中HPV DNA检测及致病性的研究

Detection and Pathogenicity of Human Papillomavirus-DNA in the Vapor of Wares Treated CO_(2)Laser

目的 探讨CO2激光治疗尖锐湿疣过程中所排放的烟雾是否有人类乳头瘤病毒（HPV DNA）存在，这些病毒的致病性如何。方法 收集32例尖锐湿疣患者的烟尘标本共96份，距烧灼部位1cm处、3cm处、5cm处各32个；用PCR方法检测烟尘标本中的HPV DNA，观察其阳性率；用烟尘标本涂擦30只大白鼠的阴道、外阴粘膜，对有皮损的部位做病理切片及PCR检测。结果 用CO2激光治疗尖锐显疣的过程中，有HPV DNA存在。经大白鼠感染试验，有一定的致病性。结论 用CO2激光治疗的烟尘中有HPV DNA存在；烟尘中的HPV DNA有一定的致病性；排烟设备距烧灼部位1cm以下最为安全。

Objective In order to study whether there are human papillomaviruses (HPV) in the vapor during CO 2 laser treatment of Condyloma acuminatum .Methods The vapor specimens were collected 1?cm(32 samples),3?cm(32 samples) and 5?cm (32 samples) from the laser treated area. The vapor specimens were detected for HPVDNA by polymerase chain reaction (PCR) methods. Observing the pathogenesis of these vapor specimens by daubing vagine and vulvae of 30 mouse. Results The results showed that HPVDNA were found in the vapor during treating condyloma acuminatum by high power CO 2 laser. The results of animal testes showed that there is pathogenesis to operator in the vapor during CO 2 laser treatment of condyloma acuminatum. Conclusion HPVDNA were found in the vapor during treating concluloma acuminatum by high power CO 2 laser. There is pathogenesis to operator in the vapor during CO 2 laser treatment of condyloma acuminatum. The equipments of draming off vapor was 1?cm from the laser treated area is safe.