空间和地面条件下斑头雁血红蛋白晶体结构的比较研究

Comparison of Structures between Crystals of Bar-headed Goose Hemoglobin Grown in Space and Earth

在 1994年我国返回式卫星FSW 2上进行的空间蛋白质晶体生长实验中 ,获得了适合于X射线分析的空间实验组和地面对照组的斑头雁血红蛋白晶体 ,并收集了X射线衍射数据。应用分子置换法解析了结构 ,并进行了比较研究。结果显示空间晶体的分子间和分子中亚基间的相互作用趋于减弱 ,在α1β2 “开关区”比地面晶体多一个α1Thr4 1CG2与 β2 His97ND1的接触。

During the experiment of protein crystal growth in space carried by Chinese re entry capsule FSW 2,the crystals of Bar headed Goose hemoglobin suitable for X ray analysis were obtained both in space and earth as contrast group.The diffraction data of both crystals were collected,and the structures were solved using molecule replacement methods.The comparison of structures in space and earth indicate that,(1) The interactions among molecules in crystal and subunits of molecule in space is weakened relative to the crystal on earth,(2) There is a contact in the α 1β 2 “switch region” of molecule in space,between α 1Thr41CG2 and β 2His97ND1,which doesnt appear in earth.