N-乙酰半胱氨酸对暴发性肝功能衰竭动物血浆氨基酸的影响

The Effects of N-acetylcysteine on Plasma Amino-acids in Animals With Fulminant Hepatic Failure

目的 :研究N -乙酰半胱氨酸对动物暴发性肝功能衰竭的治疗作用与血浆氨基酸含量的关系。方法 :采用邻苯二甲醛柱前衍生、反相高效液相色谱和荧光检测测定血浆氨基酸含量。结果 :N -乙酰半胱氨酸能降低暴发性肝功能衰竭动物血浆主要氨基酸含量 ,提高支链氨基酸/芳香氨基酸比值 ,降低动物死亡率。结论 :N -乙酰半胱氨酸治疗暴发性肝功能衰竭与其影响血浆氨基酸含量有关。

OBJECTIVE:To investigate the relationship between therapeutic effects of N-acetylcysteine(NAC) on animals with fulminant hepatic failure and contents of plasma amino-acids METHODS:Plasma amino-acids were analysed as their precolumn derivatives with O-phthol-dialdehyde by reversed-phase high-performance liquid chromatographic separation and fluorescence detection RESULTS:NAC could decrease the contents of plasma amino-acids,increase the ratio of BCAA/AAA and decrease the mortality of fulminant hepatic failure animals CONCLUSION:Therapeutic effects of NAC on animals with fulminant hepatic failure were related to its effect on the contents of plasma amino-acids