产免疫调节活性多糖海洋放线菌的筛选

Screening of marine actinomycetes producing polysaccharides with immunomodulating activity

用硫酸苯酚法和实验动物模型检测分离于厦门海区潮间带的 996株海洋放线菌胞外多糖产量和体内外免疫增强活性 .结果表明 ,3 3%的海洋放线菌粗多糖产量大于 3g/dm3;在粗多糖产量高的海洋放线菌中有 3株菌株的胞外多糖在体内外均具有较好的免疫增强活性 ,其中链霉菌 (Streptomycessp .) 2 30 5菌株的胞外多糖具有较高的非特异性、细胞及体液免疫增强活性 .研究结果表明海洋放线菌胞外多糖是免疫调节剂开发的重要资源 .

The purpose of this work is to investigate the production of the extracellular polysaccharides(EPS) with immunomodulating activity from marine actinomycetes. 996 strains of marine actinomycetes are isolated from intertidal zone of Xiamen Island, and 3.3% of them yield EPS more than 3 g/dm 3 when determined by method of phenol-sulfuric acid. Among the strains with high yield of EPS, there are 3 strains those produe the EPS having immunomodulating activity in vitro by promoting the proliferation and accumulation of interleukin 2 (IL-2) of lymphocytes of mouse spleen and in vivo in the animal model. The EPS produced by Strain 2305, Streptomyces sp., is the most promising candidate to develop into immunomodulator in view of its obvious effects on the non-specific, cellular and humoral immunity. The results show that the EPS from marine actinomycetes can be an important resource for the development of immunomodulators.