肝癌组织中TIMP-1和TIMP-2的表达意义

TIMP-1 and -2 related antigens expression in liver carcinoma tissues

目的 了解 TIMP- 1和 TIMP- 2在肝癌组织中的表达状态 ,探讨 TIMP- 1和 TIMP- 2在肝癌组织生长、侵润及转移中所起的作用 .方法 以抗 TIMP- 1和 TIMP- 2单克隆抗体 (m Ab)为试剂 ,采用免疫组织化学法检测原发性肝癌、肝高分化腺癌的肝组织中 TIMP- 1和 TIMP- 2的表达 ,并与 10例正常肝组织做对照 .结果 10例原发性肝癌患者的肝组织中TIMP- 1和 TIMP- 2蛋白表达的阳性率为 10 0 % ,在癌组织及非癌组织中均有表达 .癌组织中的 TIMP- 1和 TIMP- 2蛋白表达的强度比较为 6例高于、4例低于周围的非癌组织 (慢性肝炎及肝硬化组织 ) ,癌组织中 TIMP- 1和 TIMP- 2蛋白的表达呈散在性分布 ,但 TIMP- 1比 TIMP- 2表达强 .9例肝高分化腺癌的腺癌组织中无 TIMP- 1和 TIMP- 2相关抗原的表达 ,但癌周组织的肝细胞有 3例 TIMP- 1和 TIMP- 2蛋白表达为阳性 .10例正常肝组织中 TIMP- 1和 TIMP- 2蛋白表达均为阴性 .结论 TIMP- 1和 TIMP- 2存在于原发性肝癌的癌组织中 ,其表达的部位与强度可能与癌的生长、浸润及转移有关 .

AIM To investigate the distribution of tissue inhibitor of metalloproteinasas 1 (TIMP 1) and TIMP 2 in liver carcinoma tissues and discuss the function of TIMP 1 and TIMP 2 for growth, invasion, and metastasis of carcinomas of liver. METHODS The cellular distribution of TIMP 1 and TIMP 2 was studied in surgically removed human hepatocellular carcinomas and well differentiated adenocacinoma of liver samples by immunohistochemical method using monoclone antibody (mAb). RESULTS TIMP 1 and TIMP 2 protein were detected in all 10 samples of the hepatocellular carcinomas. Both TIMP 1 and TIMP 2 were positive in the cancer cells and noncarcerous cells, and the expression of TIMP 1 was stronger than that of TIMP 2. The expression of TIMP 1 and TIMP 2 in 6 samples was higher, and that in other 4 samples was lower than that in the surrounding noncancerous cells (chronic active hepatitis and liiver cirrhosis). The distribution of TIMP 1 and TIMP 2 in the cancer cells was dispersed. TIMP 1 and TIMP 2 protein were not detected in the well differentiated adenocarcinomal of liver. Among the 9 samples, TIMP 1 and TIMP 2 protein were positive in the surrounding noncancerous liver tissue of 3 samples (chronic active hepatitis). None of normal liver tissue is positive. CONCLUSION Both expression of TIMP 1 and TIMP 2 in primary carcinomas of liver may be related with the growth, invasion and metastasis of primary carcinomas of liver.