逆转录酶抑制剂对卵巢癌细胞株HO-8910细胞生长的影响

Effect of 3'-azido-3'-deoxythymidine on the growth of HO-8910 cell line

目的 探讨逆转录酶抑制剂 (AZT)对卵巢癌细胞HO- 8910生长的影响及其作用机制 .方法 应用 MTT分析法对不同浓度 AZT作用的卵巢癌 HO- 8910细胞进行药物敏感实验 ,流式细胞仪测定凋亡细胞含量 .结果 AZT对 HO-8910细胞的生长有抑制作用 ,并有时间依赖关系和剂量依赖关系 .流式细胞仪检测凋亡峰含量为 14.2 % .结论 AZT可能是通过诱导凋亡而抑制卵巢癌细胞的生长 。

AIM To investigate the effects of 3 azido 3 deoxythymidine (AZT) on HO 8910 cell line and its mechanism. METHODS The drug sensitivity test was performed with MTT assay. The apoptotic cells were measured with flow cytometry (FCM). RESULTS AZT inhibited growth of HO 8910 cell and the effect was in a time dependent manner and dose dependent manner. The apoptotic peak was detected with FCM and the quantity was 14.2%. CONCLUSION It may be through inducing apoptosis that AZT exerts inhibitive effect on ovary carcinoma cells growth. Our results indicate that AZT has great potential for the treatment of ovary carcinoma.