动脉粥样硬化遗传易感性与YNZ22多态性的相关性

Relationship between YNZ22 VNTR polymorphism and atherosclerosis suceptibility

目的 研究 YNZ2 2基因多态性在陕西地区老年动脉粥样硬化 (atherosclerosis,AS)人群中分布规律及其该位点与 AS遗传易感性的关系 .方法 应用多聚酶链反应(polymerase chain reaction,PCR)对 94例老年 AS患者及 75例非 AS老年人 YNZ2 2 - VNTR(可变数目串联重复序例variable number tandem repeats)位点进行多态性分析 .结果 在正常人群中 YNZ2 2位点共检测出 2 0种基因型 ,9个等位基因 ,其片段大小在 16 8～ 798bp之间 ,杂合度为 2 4.0 % ,多态信息量 (polymorphism inform ation content,PIC)为 0 .8372 ;AS人群中 YNZ2 2基因点共检测出 2 3种基因型 ,9个等位基因 ,其片段大小在 16 8～ 72 8bp之间 ,杂合度为 2 4.5 % ,多态信息量 (PIC)为 0 .80 6 7.它们的基因频率分布有差异 .另外 ,在正常人群与 AS人群中 ,小片段的频率有显著差异 .最重要的是发现基因频率和基因型频率在两群体中不同 .结论 YNZ2 2位点可能可以作为

AIM To observe the polymorphism of YNZ22 gene on atherosclerosis people in Shaanxi province and toassess the possible association between polymorphism and suceptibility of atherosclerosis so as to provide clues for genetic marker of atherosclerosic. METHODS Polymorphism at YNZ22 VNTR locus was studied by PCR in 94 cases of atheroclerosis and 75 cases of normal old people of Shaanxi. RESULTS 20 genotypes, 9 alleles ranging from 168 bp to 798 bp were revealed in normal old people of Shaanxi. The heterozygosity of YNZ22 was 24.0%, PIC 0.9372; 23 genotypes, 9 alleles ranging from 168 bp to 798 bp were revealed in atherosclerosis cases. The heterozygosity of YNZ22 was 24.5%, PIC 0.8067. There was difference in distribution of allele frequency; and obviously between atherosclerosis and normal people in allele and genotypes frequency. CONCLUSION YNZ22 locus can be the polymorphic genetic marker of the atherosclerosis.