定心方对大鼠缺氧及正常心肌细胞内钙、膜电位和线粒体膜电位的影响

Effect of Dingxin Recipe on Intracellular Free Calcium, Membrane Potential and Mitochondria Membrane Potential in Normal and Hypoxia Myocytes of Rats

目的 :探讨定心方 (DXR)抗心律失常的分子机理。方法 :采用胰蛋白酶法分离培养心肌细胞 ,用不同荧光染料分别标记细胞 ,在激光共聚焦显微镜上测定DXR血清对心肌细胞内钙〔Ca2 + 〕i、膜电位(MP)和线粒体膜电位 (MMP)的变化。结果 :缺氧使心肌细胞〔Ca2 + 〕i和MMP升高 ,而使MP降低 ,DXR血清降低了正常和缺氧心肌细胞〔Ca2 + 〕i,改善了缺氧引起的MP降低 ,使缺氧状态下MMP保持在基线水平。结论 :DXR能通过抑制缺氧引起的〔Ca2 + 〕i和MMP升高 ,及拮抗缺氧所致的MP降低 ,从而发挥保护心肌细胞 ,防治心律失常的作用。

Objective: To elucidate the molecular mechanism on anti arrhythmic function of Dingxin Recipe (DXR). Methods: Cardial myocytes isolated by trypsin method were cultured and labelled with various flourescence stains. DXR contained serum induced changes of cytolic calcium concentration 〔Ca 2+ 〕i, cell membrane potential (MP) and mitochondria membrane potential (MMP) were determined with laser confocal microscopy. Results: Hypoxia caused increase of 〔Ca 2+ 〕i and MMP, and decrease of MP, while DXR contained serum lowered the 〔Ca 2+ 〕i in normal or hypoxian myocytes, improved hypoxia induced MP reduction and maintain MMP at baseline level under hypoxia circumstances. Conclusion: Through inhibiting the hypoxia caused 〔Ca 2+ 〕i and MMP elevation and MP lowering, DXR displays its action in protecting myocardial cells to prevent and treat arrhythmia.