应用噬菌体裂解法快速鉴定结核分支杆菌

Application of rapid detection for Mycobacterium tuberculosis with phage splitting assay

目的 研究噬菌体裂解试验对结核分支杆菌快速鉴定的意义。方法 应用结核分支杆菌噬菌斑技术快速检测结核分支杆菌、非结核分支杆菌和非分支杆菌及肺结核患者痰标本。结果 结核分支杆菌H37Rv、牛分支杆菌和非洲分支杆菌噬菌体裂解试验均为阳性 ,10株常见非结核分支杆菌和 7株非分支杆菌均为阴性 ;30株结核分支杆菌临床分离株本法检测结果全部阳性 ;2 0份涂阳培阳肺结核患者痰标本 ,本法阳性 19份 (95 % ) ;2 1份涂阴培阳痰标本 ,本法阳性 15份 (71% ) ;19份涂阴培阴痰标本 ,5份阳性 (2 6 % )。结论 噬菌体裂解试验可以快速鉴定结核分支杆菌 。

Objective To study the significance of rapid identification for Mycobacterium tuberculosis with phage splitting assay Methods Strains of Mycobacterium tuberculosis ,non tuberculosis mycobacterium,non mycobacterium and samples of sputum with pulmonary tuberculosis were rapidly detected by phage spot technique Results The strains of Mycobacterium tuberculosis H 37 R v,bovis and africanum were all positive. The results of 10 strains of non tuberculosis mycobacterium and 7 strains of non mycobacterium were negative. All of 30 clinical isolates from the patients of the pulmonary tuberculosis were positive. 19 of 20 sputum specimen of pulmonary tuberculosis,which were all positive detected by smear and culture,were positive. There were 15 specimen positive in 21 sputum with negative tested by smear and positive by culture. Besides,5 of 19 sputum specimen with negative by smear and culture were positive detected by this method.Conclusion The phage splitting assay can be used for rapid identification of Mycobacterium tuberculosis ,which possesses high specificity and sensitivity for detection of Mycobacterium tuberculosis in sputum specimen.