用微管吸吮技术测定丹皮酚对红细胞变形性的影响

The Effect of Paeonol on Erythrocyte Deformability Determined by Mcropipette Aspiration Technique

目的 用优化的微管吸吮技术测定丹皮酚对红细胞变形性的影响,为推广应用微管吸吮技术提供实验依据。方法:采用微管吸吮实验技术对大鼠体内用药后的红细胞进行测定,简化了红细胞变形性的计算方法,提出了表观变形指数ADI,并与对照组进行统计学分析。结果:红细胞变形指数计算简便、判断直观。丹皮酚的变形指数ADI_(min)、ADI_(max)以及最大变形时间T_(max)与对照组相比具有显著差异。结论:丹皮酚对红细胞的变形性有显著的影响,同时实验也证明微管吸吮法用于测定中药对红细胞变形性的影响不失为一种快捷而实用的方法。

Objective: The effect of Paeonol (PAE) on erythorcyet(RBC) deformability was studied in order to provide experimental data for the application in clinic and the evaluation in pharmacological action. Methods: Micropipette Aspiration Technique was used to determine the RBC deformation length. The method of calculation was also simplified and a new apparent deformation Index( ADI) was difined. Results: PAE could increase the ADImin and ADImax and decrease Tmax significantly. Conclusion: PAE has the effect of deformational ability on RBC. Micropipette aspiration technique is a feasible method for determining the effect of traditional Chinese medicine on erythrocyte deformability.