香荷药条的质量标准研究

Study on the Quality Standards for Xianghe Suppository

目的:研究香荷药条的质量标准,建立黄连、苦参、丁香的薄层色谱鉴别方法和小檗碱、巴马亭的高效液相色谱含量测定方法;方法:流动相为乙腈-水(1:1,1000ml含KH2PO43.4 g,CH3(CH2)11SO4Na1.7 g),检测波长345nm,样品提取过滤后进行分析;结果:回归方程、相关系数及线性范围分别为:小檗碱Y=1.41×105X+5.46×106,r=0.9999,线性范围0.07～2.24μg;巴马亭Y=1.96×105X+6.81×106,r=0.9997,线性范围0.07～2.24 μg;结论:该方法适用于香荷药条的质量控制.

The quality standards for Xianghe suppository were studied. The TLC methods for identification of Rhizoma Coptidis, Radix Sophorae Flavescentis and Flos Caryophylli were established . A simple high performance liquid chromatographic method was established for de-termination of berberine and palmatine. The mobile phase was acetonitrile-water( 1:1, containing KH2PO4 3.4 g, CH3 ( CH2) 11 SO4Na 1.7 g per 1000 ml). UV detecting wavelength was 345 nm. This method is suitable for the quality control of Xianghe suppository.