高效液相色谱法测定戊菌隆在棉花及土壤中残留

Determination of Pencycuron Residues in Cotton and Soil by HPLC

试样采用二氯甲烷提取 ,弗罗里硅土和中性氧化铝层析柱净化 ,高效液相色谱仪检测戊菌隆在棉叶、棉籽及土壤中残留量。最小检知量为 5× 10 - 1 0 g ,最低检知浓度 :土壤 0 0 2 5mg/kg ,棉叶、棉籽 0 0 5mg/kg。添加回收率 :土壤 90 5 %～ 90 6 % ,棉籽 87 6 %～ 94 4% ,棉叶 86 5 %～88 8% ;变异系数 :土壤 2 1%～ 3 5 % ,棉籽 2 2 %～ 6 7% ,棉叶 1 1%～ 3 2 %。

Residual pencycuron in cotton and soil were extracted by dichloromethane,cleaned up by passing through a magnesium silicate-aluminum oxide(Neutral)column,determined by HPLC The detection limit was as low as 5×10 -10 g,and the minium detectable amount in cotton and soil were 0 05mg/kg and 0 025mg/kg ,the average recoveries of pencycuron in soil and cotton leaves and seeds were 90 5%～90 6% and 86 5%～88 8% and 87 6%～94 4% respectively,the coefficients of veriation ranged from 2 1% to 3 5% and 1 1% to 3 2% and 2 2% to 6 7%