TECA型生物人工肝脏治疗少肝急性肝衰犬的效应

Effect of TECA bioartificial liver support system on the canines with acute liver failure by partial liver resection

目的 :观察TECA型生物人工肝支持系统 (BALSS)治疗少肝急性肝衰 (ALF)犬的安全有效性。方法 :以肝脏切除 80 %诱发犬ALF。采用含中国实验用小型猪肝细胞的BALSS对ALF犬血进行 6h交叉灌流 (肝细胞组 ) ;以无肝细胞组及药物组作为对照治疗 ;测定治疗前、后ALF犬血生化、免疫学指标 ,观察犬脏器组织学变化。结果 :BALSS治疗后 ,肝细胞组 10只ALF犬的血NH3、BIL、ALT、AST明显下降 (P <0 0 1) ,PT、PA明显改善 (P <0 0 5 ) ;犬平均生存期为 (10 8 0± 12 0 )h ,其中 3只犬存活 >30d ;其免疫学指标无明显改变。而不含肝细胞的透析及常规药物治疗不能改善ALF犬的上述生化指标 ;其治疗后的平均生存期分别为 (2 4 0± 6 0 )h、(2 0 4± 6 4)h。结论

Objective:To evaluate the efficacy and safety of the TECA BALSS in treating ALF canines induced by partial liver resection Methods:10 ALF canines received BALSS treatments,and 4 ALF canines were treated with BALSS without porcine hepatocytes Blood was perfused through a hollow fiber tube containing 1×10 10 porcine hepatocytes 5 AlF canines received drugs Each treatment lasted 6 h.Results:ALF canines got beneficial effects with decreased the level of plasma ammonia, ALT, AST, BIL, PT and increase level of PA after BALSS treatment There were no changes in count of lymphocytes, immune proteins (IgA, IgG, IgM) and supplementary (C3, C4) levels after BALSS treatment In contrast, for the ALF canines in non hepatocyte BALSS group and drug group,there were no significant changes in levelsof ammonia, BIL, ALT, AST, BIL, PT and PA ALF canines in BALSS group,non hepatocyte BALSS group and drug group lived respectively an average time of 108 0±12 0 h,(24 0±6 0) h and (20 4±6 4) h,and 3 ALF canines in BALSS group survived. Conclusion:TECA BALSS is efficacious and safe for ALF canines induced by partial liver resection.