血啉甲醚和血卟啉衍生物在类风湿关节炎滑膜细胞中吸收特性的对比研究

Comparison of uptaking characteristics of HMME and HpD by synovial cells of rheumatoid arthritis in vitro

目的 :探讨体外培养的类风湿关节炎 (RA)患者的滑膜细胞对光敏剂血啉甲醚 (HMME)的吸收特点 ,并与目前常用的光敏剂血卟啉衍生物 (HpD)进行对比。方法 :用荧光分光光度计法检测RA滑膜细胞对HMME、HpD的吸收量与孵育时间和孵育浓度的关系。结果 :①RA滑膜细胞对HMME、HpD的吸收量随着孵育浓度的增加而增加。当培养液中光敏剂浓度达 80 μg/ml时 ,孵育 1h细胞内HMME含量达到饱和状态 ;而HpD的浓度要到 140μg/ml时细胞内的吸收量才达到高峰 ,当两种光敏剂细胞内浓度均达高峰时 ,HMME的含量为HpD含量的 2倍。②细胞对两种光敏剂的吸收量亦随着孵育时间的增加而增加 ,HMME吸收得更快 ,第 15分钟时吸收量为最大吸收量的 5 2 9% ,第 6 0分钟为 84 4% ,细胞对HpD的吸收第 15分钟为 49 1% ,第 6 0分钟为 6 9 2 %。结论 :与光敏剂HpD相比 ,RA滑膜细胞能更多更快地吸收HMME。

Objective:To investigate the uptaking characteristics of hematoporphyrin monomethyl ether (HMME) by synovial cells of RA in vitro And compared with hematoporphyrin derivative (HpD), the traditional photosensitizer.Methods:The spectrofluorometer was used to quantitate the concentrations of HMME and HpD in cultured RA synovial cells The relationship between the cellular photosensitizers contents and the concentrations in the medium and the incubating time were determined.Results:The uptake of HMME by RA synovial cells increased with the elevation of the HMME concentration in the medium The cellular HMME content exhibited a saturation pattern, reaching a plateau when exposed to 80 g/ml HMME for 1 hour The saturation concentration of HMME was twice than that of HpD Cellular uptake of HMME was rapid and shown to be time dependent, with 52 9% of maximum concentration reached within 15 minutes, and 84 4% within 60minutes While HpD were 49 1% and 69 2%, respectively. Conclusion:The cultured synovial cells of RA could rapidly uptake more HMME than HpD It was concluded that HMME was superior to HpD in photodynamic synovectomy.