丙肝宁冲剂中大黄与虎杖的定性定量研究

Qualitative and Quantitative Determination of Radix et Rhizoma Rhei and Rhizoma Polygoni Cuspidati in Bingganning Granules

目的 :建立丙肝宁冲剂的部分质量标准。方法 :采用TLC法对方中大黄、虎杖进行定性鉴别 ,采用HPLC法测定虎杖中白藜芦醇苷的含量。结果 :大黄中的芦荟大黄素可作为其定性依据。白藜芦醇苷含量测定平均回收率为 97.0 5 % ,RSD为 1.6 2 5 % ,暂定标准为不少于 4mg/ g。 结论 :此法简单快捷 。

Objective: To establish the part of quality control standards for Bingganning Granules. Methods: Radix et Rhizoma Rhei and Rhizoma polygoni cuspidati in Binggamning Granules were identified by TLC and polydatin of Rhizoma polygoni cuspidati was determined by HPLC. Results: The content of aloe emodin in Radix et Rhizoma Rhei could be used as a basis of quantitation. The average recovery of polydatin was 97.05%. RSD was 1.625%. The content of polydatin in Bingganning Granules was fixed at least not to low than 4mg/g. Conclusion: The method is simple, quick, accruate and with good reproducibility.