银杏总黄酮苷对鸡眼形觉剥夺影响的实验研究

Experimental Study on Effect of Ginkgo Biloba Extract on Form-Deprivation Myopia in the Chick

目的 :观察银杏总黄酮苷对鸡眼形觉剥夺的影响。方法 :2d龄鸡雏 30只 ,分 3组 ,分别为对照组、用药Ⅰ组、用药Ⅱ组 ,每组均缝合单只眼睑 ,造成形觉剥夺近视眼。采用测定鸡眼屈光度、活体A超眼轴测量、光镜下观察巩膜、视网膜病理形态的方法。结果 :形觉剥夺导致了对照组近视性屈光状态异常 (P <0 .0 0 1)。眼球的前后轴长度延长 (P <0 .0 5 ) ,光镜下巩膜软骨细胞层增厚 ,巩膜纤维层变薄。用药组近视性屈光状态异常有改善。眼球前后轴长度缩短 ,其中用药Ⅱ组的屈光度与眼前后轴长度缩短与对照组比较 ,差异具有统计学上的显著意义 (P <0 .0 5 )。镜下用药Ⅱ组近视眼巩膜软骨细胞、巩膜纤维层改变不明显。结论 :研究结果提示 ,银杏总黄酮苷对鸡眼形觉剥夺后的近视性屈光状态形成及眼球前后轴长度有抑制作用 。

Objective: To observe the effect of Ginkgo Biloba extract (EGb) on Form deprivation Myopia (FDM) in the chick. Methods: 30 two day chicks were divided into three groups (Control Group, Drug Group Ⅰ, Drug Group Ⅱ). All of them were monocularly deprived by suturation of eyelids. Axial length was measured by A Scan ultrasonograph, the posterior cartilaginous sclera, the sclera fibrous and retina were observed under the light microscope. Results: Both refraction and axial eye length were changed by form deprivation in control group. refraction change ( P <0.001), axial eye length change( P <0.05); Sclera cartilage cell layer was increased, Sclera fibrous layer were decreased. In the drug groups, the chick refraction changes have improved, The axial eye length have been contracted especially in the drug group Ⅱ( P <0.05). Under the light microscope, FDM drug group, the change of sclera cartilage and fibrous layer are not obvious. Conclusion: The Myopia refraction and axial eye length of FDM in the chick can be inhibited by EGb. The change of sclera cartilage and sclera fibrous layer can be improved by EGb.