食管癌血清和尿液中血管内皮生长因子水平的变化及其意义

Change in vascular endothelial growth factor level in serum and urine of esophageal carcinoma patients

目的 检测食管癌患者术前血清和尿液中血管内皮生长因子 (VEGF)水平 ,探讨VEGV与食管癌的关系及其临床意义。方法 应用酶联免疫吸附法 (ELISA)对 82例食管癌患者术前血清和尿液中VEGF水平进行测定 ,并对其进行对照分析。结果 食管癌患者术前血清和尿液VEGF水平分别为 (92 .81± 5 .86 )ng/L和 (2 77.92± 2 2 .71)ng/L ,明显高于正常人组 (P <0 .0 1)。食管癌组术前尿液VEGF水平与肿瘤的分化程度、侵袭程度、临床分期及淋巴结转移等密切相关 (P <0 .0 5 ) ;且术前尿液组的VEGF水平明显高于术前血清组 (P <0 .0 1)。结论 VEGF可能参与食管癌的发生、发展和转移过程的调控 ,尿液VEGF含量的增强 ,预示食管癌的转移和预后不良。

Objective The purpose of this study was to determine the serum and urine vascular endothelial growth factor (VEGF) level in patients with esophageal carcinoma and to elucidate their significance of pre operative VEGF levels. Methods Urine VEGF level was measured by sandwich ELISA in 82 esophageal carcinoma patients and 15 healthy individuals.Results The serum and urine VEGF levels ( ±s, ng/L) in esophageal carcinoma patients were 92.81±5.86 and 277.92±22.71 which were significantly higher than those of healthy subjects (16.01±3.46 and 15.27±4.22, P <0.01). The pre operatiive urine VEGF level in esophageal cancer patients were significantly higher in advanced stage of the disease ( P <0.01). As to the depth of invasion (T factor), the urine VEGF level of T4 invasive lesions were significantly higher than those with T1 T3 invasion ( P <0.01). The same was also true in esophageal carcinoma having lymph node metastasis than that without ( P <0.05). The urine VEGF level was significantly higher than the serum VEGF level ( P <0.01).Conclusion VEGF plays an important role in the growth and metastasis of esophageal cancer. [