转bak基因协同阿霉素杀伤膀胱癌耐药细胞

Bak gene cooperating with adriamycin to kill drug-resistant bladder cancer cells

目的 :研究转 bak基因与阿霉素 (ADM)联合杀伤膀胱癌耐药细胞的效果 ,并探讨其机制。方法 :利用脂质体将基因 bak导入膀胱癌耐药细胞 ,通过原位杂交法检测 bak m RNA的表达 ,同时加入 ADM,利用 MTT法算出细胞生存率 ,进而推算半数抑制浓度 (IC50 )和抗药指数。用荧光分光光度计检测细胞内 ADM的聚集量。结果 :转基因后 ,细胞内 bak m RNA阳性表达、细胞生存率较单用 ADM明显降低 (P<0 .0 5 ) ,细胞内 ADM聚集量无明显增加 (P >0 .0 5 )。结论 :转 bak基因可以协同 ADM更有效地杀伤膀胱癌耐药细胞 ,但并非通过帮助 ADM进入细胞起作用。

Purpose:To study the effects of killing drug resistant bladder cancer cells by the use of transferred bak gene and adriamycin and illustrate its mechanisms.Methods:Bak gene was transfected into drug resistant bladder cancer cells by liposome. The expressions of bak and bcl 2 were detected by in situ hybridization. The survival rate was worked out by MTT after adriamycin was added in and IC 50 and resistant factor were calculated. The accumulation of adriamycin in the cells were examined by spectrofluorometry.Results:The expression of bak mRNA was positive in EJ/bak cells. The survival rate of EJ/bak cells was significantly decreased compared with the cells in which adriamycin was added (P< 0.05 ). The accumulation of adriamycin in the cells did not increase significantly.Conclusions:The use of transferred bak gene and adriamycin could kill drug resisant bladder cancer cells more efficiently. But bak gene did not operate through helping adriamycin into cells.