摘要
目的 :探讨三氧化二砷 (As2O3)诱导人胃腺癌细胞SGC7901的凋亡效应以及细胞线粒体跨膜电位 (ΔΨm )的改变。方法 :通过形态学观察以及测定细胞活力、亚G1 期细胞含量 ,细胞膜磷脂酰丝氨酸 (PS)外翻量 ,罗丹明123(Rh123)/碘化丙啶 (PI)双染色 ,流式细胞仪检测ΔΨm变化等方法鉴定细胞凋亡。结果 :As2O3 诱导细胞凋亡效应与其诱导ΔΨm下降密切相关。结论 :细胞ΔΨm下降是As2O3 诱导肿瘤细胞凋亡的机制之一。
OBJECTIVE:To investigate the possible role of the alteration of mitochondrial transmembrane potentials(ΔΨm) in arsenic trioxide(As2O3)-induced apoptosis of human gastric adenocarcinoma cells(SGC7901) METHODS:Cell apoptosis was assessed through morphology,cell viability,the percentage of sub-G1 cells and phosphatidylserine(PS) externalization the ΔΨm was detected on flow cytometry through double staining of Rhodamine 123 (Rh123) and propidium iodide(PI) RESUL_TS:The As2O3-induced apoptosis was associated closely with the disruption of the ΔΨm CONCLUSION:The disruption of the ΔΨm is one of the mechanisms of As2O3-induced apoptosis
出处
《中国药房》
CAS
CSCD
2001年第6期333-334,共2页
China Pharmacy
关键词
三氧化二砷
胃腺癌
凋亡
线粒体
arsenic trioxide
gastric adenocarcinoma
apoptosis
mitochondria
