血液中全铁的氧化电位溶出法测定

Study on the Determination of Iron by Differential Potentiometric Stripping Analysis

在 0 .0 5mol·l-1磺基水杨酸 - 0 .4mol·l-1醋酸钠溶液中 ,Fe3+在 - 1.5V(vs.SCE)的汞膜玻碳电极上搅拌电解富集 5min ,静止电解富集 1min后 ,靠溶解氧氧化溶出 ,在 - 0 .74V处得到铁的微分电位溶出峰 ,由此首次建立了铁的氧化电位溶出法。在 0 .0 5～ 4.0 μg·ml-1范围内 ,微分电位溶出峰高与铁的浓度有很好的线性关系 ,检测限为 0 .0 5 μg·ml-1。将该方法应用于血液中全铁的测定 。

A new method for the determination of iron by differential potentiometric stripping analysis was proposed. In 0.05?mol·L -1 sulfosalicylic acid-0.4 mol·L -1 sodium acetate medium, Fe 3+ was stirringly deposited for 5?min , and then statically deposited for 1 min at -1.5?V(vs. SCE)on a glass carbon electrode with a mercury film, and finally stripped oxidizedly by dissolved oxygen at -0.72?V(vs. SCE). The stripping peak height of iron is proportional to the concentration of iron in a range of 0.05～4.0?μg·ml -1 Fe, and the detection limit of the method is 0.05?μg·ml -1 Fe. The method was applied to the determination of iron in whole blood with satisfied result.