测定金鸡纳碱的流动注射化学发光新体系

A New Sensitive Chemiluminescence System for Determination of Cinchona Alkaloids with FIA

过氧化氢和高碘酸钠溶液混合能产生弱化学发光 ,在这一体系中 ,金鸡纳碱的加入能极大地增强这一化学发光强度 ,该文对这一化学发光进行了初步的探讨 ,提出了可能的发光机理 ,并据此建立了测定金鸡纳碱(奎宁、奎尼丁和辛可宁 )的高灵敏度化学发光法 ;测定奎宁、奎尼丁和辛可宁的线性范围分别为6.0×10-5 ～1.0×10-2g/L、4.0×10-5 ～1.0×10-2g/L和1.0×10-3 ～1.0×10-1g/L ,检出限分别为2.1×10-5g/L,1.4×10 -5g/L,3.5×10 -4g/L ;该法适用于奎宁药物的测定。

Weak chemiluminescence(CL)can be emitted by oxidation reaction of hydrogen peroxide with sodium periodate, CL is greatly enhanced by the addition of cinchona alkaloids (quinine, quinidine and cinchonine). The CL intensity is correlated with the concentration of each cinchona alkaloid. Based on this phenomenon, a sensitive CL method for the determination of cinchona alkaloids with FIA is described. The linear ranges of 6.0×10-5～1.0×10-2 g/L, 4.0×10-5～1.0×10-2 g/L and 1.0×10-3～1.0×10-1 g/L were obtained for quinine, quinidine and cinchonine, respectively, while detection limits of 2.1×10-5 g/L, 1.4×10-5 g/L and 3.5×10-4 g/L were achieved for quinine, quinidine and cinchonine, respectively. The method was used for the simple and rapid determination of cinchona alkaloids in pharmaceuticals. The mechanism of the sensitizing effect was also discussed.