稀土金属离子与色氨酸相互作用的荧光光谱研究

A Study on the Effect of Rare Earth Metal Ions on Fluorescence Spectra of the Tryptophan Using Fluorescence Spectroscopy

在碱性介质中 ,稀土离子与色氨酸形成离子缔合型异配位络合物使色氨酸的荧光猝灭 ,在 pH =10～11的H3BO4 HAc-H3PO4 NaOH介质中 ,色氨酸的荧光发射和稀土离子对其荧光猝灭均达到最大值 ,各个稀土离子对色氨酸的荧光猝灭强度也基本相似 ,本文还建立了稀土离子与色氨酸配合物的分子模型并对其荧光猝灭机理进行了探讨。

Rare earth metal ions and tryptophan form ton-association complex in basic medium. The complex causes fluorescence quenching of tryptophan. Fluorescence emission of tryptophan and quenching caused by rare earth metal ions both reach a climax in H3BO4-HAc-H3PO4-NaOH at pH 10 to 11, and all rare earth metal ions have the approximate effects on fluorescence quenching of tryptophan. The molecular mode of complex of rare earth metal ions and tryptophan has been founded and mechanism of fluorescence quenching has been studied in this paper.