以铽离子为荧光探针测定尿样中痕量环丙氟哌酸

Determination of Ciprofloxacin in Urine Using Terbium Ion as Fluorescent Probe

详细研究了环丙氟哌酸同稀土离子铽形成的配合物体系的荧光光谱特性及实验条件对荧光强度的影响。在 pH为 6 0的条件下 ,配合物荧光体系可发射铽离子强的特征荧光 ,其最大激发和发射波长为 3 2 8和 5 4 5nm。以稀土离子铽为荧光探针 ,测定尿样中痕量的环丙氟派酸可获得满意结果。

Factors effecting the fluorescence characteristics of the reaction CPFX-Th were studied thoroughly. The effect of the experimental conditions on the fluorescence intensity was defined. In the pH 6.0 media, CPFX-Tb complex fluorescence system can emit intrinsic fluorescence of Th. The excitation and emission wavelengths are 328 nm and 545 nm. The linear range is 1.0 x 10(-8)-1.0 x 10(-6) mol.L-1. The regression equation is F=1.57 + 4.87 x 10(8)c,r=0.999 9 with the detection limit of 5.0 x 10(-9) mol.L-1. It has been satisfactory for the determination of trace ciprofloxacin in urine by using terbium ion as fluorescent probe.