玫苯胺共振光散射法测定DNA

Determination of Deoxyribonucleic Acid with Rosaniline by a Resonance Light Scattering Method

本文研究了三苯甲烷类碱性染料玫苯胺与DNA作用的共振光散射光谱 ,在 pH =10 5～ 11的范围内 ,DNA的加入导致玫苯胺共振光散射的增强 ,在 4 85nm处 ,存在一共振光散射增强峰 ,其强度与DNA的浓度呈线性关系 ,据此建立了一种测定DNA的共振光散射法。方法的线性范围为 0～ 1 0 0mg·L-1,检测限为14 2ng·mL-1。将该方法用于混合样品中DNA的测定 ,结果令人满意。

The resonance light scattering (RLS) spectra of Rosaniline with DNA have been studied. The RLS of Rosaniline is greatly enhanced by DNA in pH range of 10.5-10.8. There is a resonance light scattering peak of at 485 nm, and the enhanced intensity of RLS at this wavelength is proportional to the concentration of DNA. The linear range of the calibration graph is 0-1.00 mg.L-1 with the correlation coefficient of 0.998 0. The detection limit is 14.2 ng.mL(-1). The relatiive standard deviation (n = 10) was within 2.1%. This method is simple, rapid and has been applied to the determination of DNA in mixed samples with satisfactory results.