比值光谱一阶导数法测定复方磺胺甲噁唑片中磺胺甲噁唑含量

Determination of Sulfamethoxazole in Compound Sulfamethoxazole Tablet by First Derivative Ratio Spectrometry

本文应用比值光谱一阶导数法测定复方磺胺甲唑片剂中磺胺甲唑含量 ,依据磺胺甲唑比值导数光谱用峰零法在 2 5 1nm处测定其含量 ,比值光谱一阶导数值与浓度 ( 1～ 15 μg·mL-1)线性关系良好 ,能有效消除甲氧苄啶的干扰 ,样品测定结果与药典方法比较没有显著差异 (P >0 0 5 )。

The first derivative ratio spectrometry is used for the determination of sulfamethoxazole in compound sulfamethoxazole tablet.According to the first derivative ratio spectrum of sulfamethoxazole,peak-zero method is applied for the determination at 251 nm.There is a good linear correlation between the first derivative ratio spectrum value and concentration (1-15 μg·mL -1 ) for the method that can eliminate the disturbance of trimethoprim,and no difference while comparing the method with pharmacopoeia method (P>0 05).