EPA和DHA在超临界流体色谱柱中的传质动力学

MASS TRANSFER KINETICS OF EPA AND DHA IN SUPERCRITICAL FLUID CHROMATOGRAPHY

用超临界流体色谱 (SFC)法研究了EPA和DHA在C18上的吸附和传质性能 .建立了色谱动力学模型并获得了模型参数 ,较好地拟合了实验流出曲线 .结果表明吸附平衡、轴向弥散。

Supercritical fluid chromatography (SFC) was used to study the adsorption equilibrium and mass transfer properties for eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) on C18 by means of impulse response technique. In order to estimate the adsorption constant and rate constants, a kinetic model was established. Chromatographic curves were calculated by using the parameters obtained from experiments, and the agreement between the experimental and calculated curves was good. It was indicated that adsorption equilibrium, axial dispersion, mass transfer and effective diffusion are important factors that influence the separation of EPA and DHA by SFC.