摘要
必需水的存在是酶在有机溶剂中保持活性的关键.本文研究了自由水初始水含量对有机溶剂中α-胰凝乳蛋白酶(α-chymotrvpsin)和嗜热杆菌蛋白酶粗品(thermoase)催化合成寡肽的影响.用无机盐水合物Na2SO4@10H2O和Na2CO3@10H2O代替自由水重复了上述反应.实验表明,足量的无机盐水合物能在有机溶剂中可逆地失去自身所带的结晶水,因而能代替自由水来提供酶所需的'必需水',并有效地控制有机溶剂中酶促反应体系的水活度(aw)值。
Essential water is very important for the enzyme to keep its bioactivity in organic solvents. The effect of free water content on the synthesis of Z - Tyr - Gly - Gly - OEt and Z - Tyr - Gly - NHNHPh by alpha -chymotrypsin and Boc - Phe - Ala - NHNHPh by thermoase in various organic solvents, including dichloromethane, cyclohexane, ethyl acetate and toluene, was investigated. By replacing free water with salt hydrates such as Na2SO4 . 10H(2)O or Na2CO3 . 10H(2)O, the effect of salt hydrates on the above reaction systems was also discussed. The results indicated that essential water can be supplied by salt hydrates, and water activity (a(w)) of the synthetic reaction system can also be fixed by salt hydrates, both favoring the formation of peptide bonds.
出处
《化学学报》
SCIE
CAS
CSCD
北大核心
2001年第10期1707-1711,共5页
Acta Chimica Sinica
基金
国家自然科学基金
关键词
蛋白水解酶
寡肽
合成
有机溶剂
酶促反应
无机盐水合物
proteinase
enzymatic oligopeptide synthesis
organic solvents
salt hydrates
essential water
