人胎脾LAK细胞活性发育动力学

Kinetics of Development of LAK Cell Activity in Human Fetal Spleens

本文采用4h^(51)Cr释放试验观察了不同眙龄脾的LAK细胞活性。胚胎发育至16周时,胎脾淋巴细胞经IL-2诱导72～96h后,产生明显的LAK活性,对Raji靶细胞的杀伤率为67.7±2.2％,而16周前(15周)的LAK活性低下(20.2±5.8％)。结果还显示,16周后的胎睥LAK活性与眙龄增长无关,与正常成人水平无显著性差异(P>0.05);胎脾LAK前体细胞的发生可能早于NK细胞。

LAK activity in human fetal spleens varied from 15-40 weeks of gestational age (gestational weeks, gw) were tested against 51Cr-labeled K562 and Raji target cells in a 4 hr 51Cr release assay. Lymphocytes from the fetal spleens that had developed to 16gw, being induced with IL-2 at 5U/ml for 72~96 hours, had sufficient LAK activity (67.7±2.2% against Raji cells). However, before 16gw, LAK activity was significantly lower (20.2±5.8%) than that after 16gw (P<0.01) .After 16gw, LAK activity did not show an age-related increase with the fetal age, and was almost same as adult levels (p>0.05) . The development of precursors of LAK cells existed in fetal spleens may be earlier than that of NK cells.