人白细胞介素2受体α链cDNA导入哺乳细胞及其克隆与稳定表达的研究

Studies on the Introduction and Stable Expression of Human Interleukin-2 Receptor a Chain cDNA in Mammalian Cells

按DNA—磷酸钙共沉淀法将人白细胞介素2受体α链cDNA转染中国仓鼠卵巢细胞(CHOdhfr^-)及小鼠成纤维细胞(L929),获得稳定表达人IL-2Rα链的CHOdhfr^+细胞克隆。经RNA点渍杂交分析、荧光标记IL-2染色法、特异性ELISA和玫瑰花环试验检测证明,哺乳细胞表达的重组IL-2Rα链具有结合IL-2和抗Tac McAb的能力。还报道了T细胞白血病Jukat及Molt-4等细胞系异常表达IL-2R的结果,并作了分析讨论。

Human interleukin-2 receptor a chain cDNA was transfected into mouse fibroblast cells (L929) and Chinese hamster ovary (CHOdhfr-) Cells by DNA-Calciu-mphosphate coprecipitalion method. Then the CHOdhfr+ clones expressing the human IL-2R a chain stably were obtained.Results from RNA dot-blot hybridization, FITC-IL-2 fluorecent staining assay and anti-Tac specific ELISA and rossette test demonstrated that the product of human interleukin-2 receptor a chain cDNA in mammalian cells is able to bind IL-2 and anti-Tac McAb. Moreover, the abnormal expression of IL-2R gene in T-cell leukemia celllines such as Jukat cells and Molt-4 cells was analysed and discussed.