淋巴因子诱导的细胞毒细胞活性检测的研究

The Research on the Detection of Cytotoxicity of Lymphokine-induced Cytotoxic Cell (LICC)

本文通过正交设计试验,建立了一种改良的诱生及检测LICC细胞毒活性的培养体系。先将脾脏淋巴细胞(5×10~6/ml)在含有IL-2(0.5U/ml)、CCDF(50％V/V)RPMI1640培养液中预培养72小时,然后加入肿瘤细胞(5×10~4/ml),用~3H-TdR后标法检测LICC细胞毒活性,杀伤时间为72小时。

Via orthogonal experiment, a modified culture system was developed, which was for incubation and detection the cytotoxic effect of LICC. At first, the sp lenic lymphocytes were preincubated for 72 hours at a concentration of 5 × 108 cell/ml in the RPMI 1640 medium supplemented with 50% CCDF and IL-2 (0. 5U/ml), and then added the EAC tumor cells (6 × 104 cells/ml) together incubation for anther 72 hours (killing time), at last, detected the cytotoxicityof LICC with 3H-TdR postla-bel.