摘要
应用mRNA麦胚无细胞体外转译体系,动态地观察了人参三醇皂甙(PTGS)对人淋巴结细胞IL-5基因表达的促进效应。结果表明,PTGS可以明显促进PHA活化人淋巴结细胞分泌IL-5,最大促进效应可达66.67％。PTGS+PHA共刺激后人淋巴结细胞浆IL-5 mRNA转译IL-5的量明显高于单纯PHA组,最大促进效应40％。上述结果首次证明PTGS对IL-5的促诱生效应是通过调节IL-5基因表达而实现的。
Promoting effect of Panaxatriol ginsenoside (PTGS) on interleukin-5 (IL-5) induction of PHA-stimulated human lymph node cells was observed. The results showed that PTGS enhanced the IL-5 production on each time of IL-5 induction kinetics, maximally by 66.67% (24h) . Furthermore, it was observed that IL-5 mRNA from PHA +PTGS-stimulated lymph node cells translated more IL-5 than PHA-stimulated lymph node cells on each time of IL-5 mRNA -induction kinetics, maximally by 40% (24h) , using the methods of wheat germ cell-free in vitro translation system and IL-5 bioassay. It is the first report on gene regulation by ginsenoside or any other kind of Chinese herbs.
出处
《免疫学杂志》
CAS
CSCD
北大核心
1991年第3期170-172,共3页
Immunological Journal
关键词
人参三醇皂甙
IL-5
基因表达
人参
Interleukin-5 mRNA, Human lymph node cells, Panaxatriol ginsenoside, Gene regulation
