摘要
我们首次将人凝血因子Ⅸ基因cDNA亚克隆于原核细胞表达载体pKK233-2,以期获得人重组凝血因子Ⅸ。实验结果,我们得到重组表达质粒pKK233-FIX1和pKK233-FIX2,以IPTG诱导,在大肠杆菌JM103中表达出一特定的、与人凝血因子Ⅸ基因cDNA表达相关的蛋白质。ELISA定性实验表明:这一特定的蛋白质具有人凝血因子Ⅸ抗原性。此项研究为最终获得人重组凝血因子Ⅸ打下了基础。
To obtain recombinant human clotting factor Ⅸ we wanted to have the factor expressed in E.coli JM103. When placed downstream from a hybrid trp-lac promoter of vector pKK233-2, in the correct orientation, the cDNA encoding the human clotting factor Ⅸ programs the synthesis of the factor-like-protein, as judged by SDS-PAGE and by ELISA.
出处
《免疫学杂志》
CAS
CSCD
北大核心
1991年第4期237-240,共4页
Immunological Journal