红棕灰夜蛾核型多角体病毒的研究

Studies on the Nuclear Polyhedrosis Virus lsolated from the Mulberry Caterpillar Sarcopolia illoba (Butler)

红棕灰夜蛾 Sarcopolia illoba(Butler)是鳞翅目夜蛾科害虫。分布于我国东北、华北、华东、华中及苏联、日本、朝鲜、印度等地。幼虫多食性,为害烟草、甘蓝、马铃薯、甜莱、大豆、豌豆、菜豆等作物。1984年在哈尔滨大豆田中,从该虫幼虫自然死虫体内分离出一株核型多角体病毒。我们对该病毒的形态学进行了观察。这种病毒在国内外尚未见报道。并对12种夜蛾科害虫:迹幽夜蛾(Discestra stigmosa)、甘蓝夜蛾(Mamestra brassicae)、黄地老虎(Scotia segetum)、小地老虎(S.ipsilon)。

This paper reports the results of studies on a Nuclear Polyhedrosis Virus(SiNPV) isolated from the mulberry caterpillar [Sacopolia illoba (Butler)]in Harbin. In most cases there were 4--5 nucleooapsids enveloped within eachvirus bundle of SiNPV. The polyhedra of SiNPV were 2. 06±0. 27μm in diameter,and nucleocapsids were measured about (0.36±0.01)×(0.058±0. 004)μm. SiNPVwas also infectious to the neonate larvae of several other noctuids includingDiscestra stigmosa, Mamestra brassicae, Scotia segetum, S. ipsilon,Autographa amurica, Macdunnoughia crassisigna, Diarsia canescens,Orthosia ella, Mythimna separata, Agrotis trifurca and Heliothis dips-acea with diffrent mortalities. When they were treated with 1.5×10~6PIB/cm^2,the motalities were all over 90%.