芽孢缺失对D-核糖产量的影响

THE EFFECT OF SPORULATING-DEFICIENT ON D-RIBOSE PRODUCING

利用原生质体紫外线诱变的方法处理转酮酶缺陷型短小芽孢杆菌 ,选育到一株丧失芽孢形成能力的转酮酶缺陷型短小芽孢杆菌 ,摇瓶培养 D-核糖平均产量达到 6 6 g/ L,较出发菌株提高 38%。研究表明 ,芽孢生成缺陷有利于 D-核糖的产生 ,筛选芽孢生成能力缺陷的菌株是选育 D-核糖高产菌株的有效手段。

Transketolase defective and D-ribose producing Bacillus pumilus stain was mutagenized by using UV after lysised to protoplast.A strain has been screened which is defective in sporulation fully and D ribose titre reached 66 g/L,D ribose titre has improved about 38%.We can conclude that screening sporulating deficient is a good method to obtain high D ribose producing strain.