CTX、G-CSF动员造血干细胞过程中NK细胞检测

The Changes of Number and Activity of Natural Killer Cells during Mobilization by Cyclophospamide and G-CSF

目的 探讨环磷酰胺 ( CTX)、粒细胞集落刺激因子 ( G- CSF)动员癌症患者造血干细胞过程中 NK细胞数量和活性的变化。方法 2 1例诊断明确的癌症患者 ,经 CTX4.0 g/m2和 G- CSF(惠尔血 ) 1 5 0 μg/d动员。动员前 (前期 )、WBC降至最低点时 (极期 )、WBC开始恢复后 3天 (恢复早期 )、WBC开始恢复后 6天 (恢复期 )用流式细胞仪计数 CD34 +细胞和 NK细胞 ,用乳酸脱氢酶释放法测量 NK细胞活性。结果 动员过程中 ,NK细胞极期显著低于前期 ,恢复期则显著高于前期 ,P<0 .0 1 ;其变化与 WBC、MNC、血小板 ( BPC)、CD34 + 细胞呈显著正相关 ,P<0 .0 5。 NK细胞活性无显著差别 ,P>0 .0 5。结论 动员过程中 NK细胞数量增加 ,活性无改变。

Objective To study the changes of number and activity of natural killer(NK) cells during the cancer patients mobilization by cyclophosphamide(CY) and recombinant human granulocyte colony stimulating factor(G CSF).Methods Peripheral blood CD 34 + cells and NK cells(CD \| 3CD + 16 CD 56 +) were counted using flow cytometry on the day before mobilization (baseline),the day of WBC nadir(nadir),3 days after the nadir(early recovery phase),six days after the nadir(recovery phase) in 21 cancer patients mobilized with CTX 4.0g/m 2,and 150ug/d G CSF(Filgrastim).NK cell activities were tested by LDH releasing method.Results Nadir NK cell numbers were significantly less and recovery phase NK cell numbers were significantly higher than that of baseline, P<0.01 .NK cell number has had positive relationship with WBC,MNC,platelet count and CD 34 + cell count during the mobilization, P<0.05 .NK cell activities had no significantly difference.Conclusion NK cell number increases during mobilization,while NK activity remains unchanged.